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Plasmid DNA binds to the spin column membrane after the cells are lysed because chaotropic agents a…
Plasmid DNA binds to the spin column membrane after the cells are lysed because chaotropic agents are added to the mixture, which changes the properties of DNA in order to favor this binding interaction. After we recover the plasmid DNA, we will cut the plasmid with restriction enzymes to remove the inserted foreign DNA and examine the ...
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