Contributed by Terence Hwa; received December 27, 2024; accepted March 21, 2025; reviewed by Vickery L. Arcus and Seppe Kuehn ...

Understanding enzyme kinetics is crucial in various scientific fields, such as biochemistry, pharmacology, and biotechnology. One of the essential components in enzyme kinetics is the maximum velocity ...

This study presents an assessment of the effect of lactate dehydrogenase (LDH) inhibition on the activity of glycolysis and tricarboxylic acid cycle. The data were collected and analyzed using solid ...

Accurate estimation of enzyme kinetic parameters is essential for enzyme engineering and industrial biocatalysis, yet their experimental measurement remains labor-intensive and costly. Although ...

Understanding the fundamental parameters of enzyme kinetics can feel like learning a new language, especially when the terms look nearly identical. If you’ve ever found yourself staring at a Michaelis ...

Division of Pre-Clinical Innovation Chemistry Technologies, National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, Maryland 20892, United States ...

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Non-Commercial (NC): Only non-commercial uses of the work are permitted. A complete ...

Understanding enzyme function in living organisms is vital for advancing biochemistry and therapeutic strategies. However, traditional in vivo enzyme assays have primarily focused on enzymes unrelated ...

Transition-state theory has provided the theoretical framework to explain the enormous rate accelerations of chemical reactions by enzymes. Given that proteins display large ensembles of conformations ...