pH is a measure of how acidic or alkaline a solution is. The diagram below shows what happens to an enzyme when denaturation occurs. Each enzyme has an optimum pH but it also has a working range of pH ...

pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable ...

This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...